OLATHE, Kans. —
Ronawk, the new biotech and biofabrication company powered by the Bio-Block™ platform, today shared highlights of new data published in the October issue of Regenerative Medicine. The article entitled “Novel Hydrogel System Eliminates and Improves Retainment of Non-Senescent Mesenchymal Stem Cell Populations”
demonstrates the significant impact of Ronawk’s hydrogel Bio-Block™ system on the cell culture process and the ability to remove the need for subculture from the process.
The research in the manuscript covers the use of Ronawk’s first developed Bio-Block™, the Tissue Block (T-Block™), which is used to successfully grow adult adipose (fat tissue) derived stem cells (ASCs).
In the manuscript, Ronawk shows they were able to successfully grow ASCs over a 6-week period without ever needing to sub-culture the ASCs which is a significant time hurdle in the cell culture process for most researchers and scientists.
The ASCs were also able to retain key stem cell features and there was a significant reduction in the number of cells that stopped dividing over time, which is known as senescence.
In addition, the paper shows that ASCs grown in the T-Blocks™ produced by-products that could be collected and used to enhance the growth of keratinocytes which are a cell type found in the skin, and which are responsible for forming the water-tight barrier that protects the rest of our skin and internal organs from the outside environment. Hence, the T-Blocks™ provide an environment, where the ACS could be more active and produce more therapeutic by-products than when grown in traditional 2D monolayers.
This work is significant because by successfully growing ASCs without the need to sub-culture, Ronawk would be able to reduce the human labor needed to grow these important cells outside of the body while maximizing the biological opportunity.
- ASCs were grown over a 6-week period without ever needing to sub-culture in Ronawk's T-Block™
- ASCs retained key stem cell features
- There was a significant reduction in the number of cells that went into senescence
- ASCs grown in the T-Blocks™ produced by-products that could be collected and used to enhance the growth of keratinocytes
- The T-Blocks™ provide an environment, where the ASCs could be more active and produce more therapeutic by-products than when grown in traditional 2D monolayers.
Link to full article in Regenerative Medicine: https://www.futuremedicine.com/doi/10.2217/rme-2022-0140?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmed
Supplemental images and video: https://www.futuremedicine.com/doi/suppl/10.2217/rme-2022-0140
Ronawk’s Bio-Block Universe™ is the first expandable Bio Platform from which biotechnology applications, processes, and technologies can be developed. Ronawk’s Bio-Block Universe™ accelerates time to result by eliminating the need for subculture in cell production which subsequently decreases contamination risk and increases cell yield. Ronawk’s Bio-Blocks™ are a customizable platform for consistent, repeatable, and scalable cell production that accelerates research into next-generation therapies.
Ronawk’s Bio-Block Universe™ transforms what was once tedious into effortless by simplifying cell culture workflows by reducing labor, consumables, and space. Ronawk's Bio-Block™ technology utilizes biomimicry of soft tissues to streamline the process of growing cells outside the body in a manner that more closely resembles how cells grow naturally in the body. This increases biological opportunities by allowing cells to build their own microenvironment while maintaining cell viability, key characteristics, and secretion of therapeutic biologics.